Definition of primary antibody

Antibodies are useful tools for the immune system to combat pathogens, but also for the biology research scientist to conduct a variety of experiments which can probe ever further into the mysteries of life. Antibodies of different isotopes, bearing unique Fc regions travel to distinct tissue compartments of the body and elicit unique responses. The removal of the Fc region to varying degrees, as in the primary antibody fragments depicted, changes the physiologic and experimental properties of the antibody. Monoclonal definition: refers to antibody molecules of singular epitopes specificity originally produced by one B-cell and sharing identical sequence. Poly clonal definition: antibody molecules which differ in their epitopes binding and complementarity region amino acid sequence, however share overall target specificity. Some antibodies require samples to be processed or treated in a specific manner. For example, many antibodies will only recognize proteins that have been reduced and denatured, presumably because this reveals epitopes that would otherwise be obscured by secondary and tertiary folding of the proteins.